The National Gender Profile for Serbia represents the first comprehensive and structured attempt to collect and analyze available secondary data across all sectors relevant to agriculture and rural livelihoods. It provides a profound analysis of gender issues in agriculture, food security, and rural livelihoods. By this, the assessment contributes to the existing body of knowledge on rural women’s status, establishes connections between gender inequalities and agriculture and food security, and sustainable development in Serbia, and suggests areas for future work to responsible stakeholders and FAO.